Data and materials availability: All data associated with this study are present in the paper or the Supplementary Materials. All other authors declare that they have no competing interests. are inventors on a patent application (WO2019126735A1) submitted by Georgia Tech Research Corporation that covers ISF collection methods presented in this study. This potential conflict of interest has been disclosed and is managed by Georgia Tech and Emory University. is an inventor of patents licensed to companies developing MN-based products, is a paid advisor to companies developing MN-based products, and is a founder/shareholder of companies developing MN-based products (Micron Biomedical). wrote the manuscript in consultation with all authors. performed data analysis and contributed to its interpretations. It bathes and surrounds the body’s cells, allowing for the delivery of materials, intercellular communication, and the disposal of metabolic waste. performed all the other analytical assays. Interstitial Fluid Definition Water, amino acids, carbohydrates, fatty acids, coenzymes, hormones, neurotransmitters, salts, and cellular products make up the fluid present in intercellular spaces. performed the mass spectrometry experiments. Author contributions: P.P.S., M.M.N., D.P.J., G.W.M., and M.R.P. MRP is also a professor of biomedical engineering at Georgia Tech and Emory University and is an adjunct professor of chemical and biomolecular engineering at the Korea Advanced Institute of Science and Technology. is currently a professor of environmental health sciences at Columbia University. is currently an assistant professor of environmental medicine and public health at the Icahn School of Medicine at Mount Sinai. ![]() is currently a clinical research scientist at Apple. Funding: This work was supported in part by the U.S. Pollack for analyzing skin histopathological samples. Ma for help analyzing samples using LC-MS and B. McAllister for helping with study design C. Overall, these studies provide a minimally invasive method to sample dermal ISF using microneedles and demonstrate human ISF as a source of biomarkers that may enable research and translation for future clinical applications. We also determined that pharmacokinetics of caffeine in healthy adults and pharmacodynamics of glucose in children and young adults with diabetes were similar in ISF and plasma. Biomarkers distinct to ISF included molecules associated with systemic and dermatological physiology, as well as exogenous compounds from environmental exposures. Because ISF does not clot, these biomarkers could be continuously monitored in ISF similar to current continuous glucose monitors but without requiring an indwelling subcutaneous sensor. Many biomarkers used in research and current clinical practice were common to ISF and plasma. Using a microneedle patch to create an array of micropores in skin coupled with mild suction, we sampled ISF from 21 human participants and identified clinically relevant and sometimes distinct biomarkers in ISF when compared to companion plasma samples based on mass spectrometry analysis. Here, we developed a minimally invasive, microneedle-based method to sample ISF from human skin that was well tolerated by participants. However, ISF has received limited attention due largely to lack of simple collection methods. Lymphangitis: infection of one or more lymphatic vessels, usually due to bacteria (case of erysipelas) and results in a red, swollen and painful course of the lymph vessel in question with an increase in the volume of the lymph node.Tissue interstitial fluid (ISF) surrounds cells and is an underutilized source of biomarkers that complements conventional sources such as blood and urine.When the lymph nodes have been removed or damaged due to cancer then the risk of lymphedema increases with the number of lymph nodes affected ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |